relative determination approach to the metabolites of protoberberine alkaloids in rat urine by liquid chromatography tandem mass spectrometry for the comparative studies on rhizome coptidis and zuojinwan preparation

نویسندگان

rui yan school of pharmacy, shenyang pharmaceutical university, shenyang, china.

qier mu school of pharmacy, shenyang pharmaceutical university, shenyang, china.

yin wang school of pharmacy, shenyang pharmaceutical university, shenyang, china.

youping liu school of pharmacy, shenyang pharmaceutical university, shenyang, china.

چکیده

the lack of authentic standards has limited the quantitative analysis of herbal drugs in biological samples. the present work demonstrated a practicable strategy for the assay of herbs and their metabolites independent of authentic standards. a liquid chromatography–electrospray ionization–mass spectrometry (lc–esi–ms) method for the qualitative and quantitative determination of the metabolites after oral administration of rhizome coptidis and zuojinwan preparation in rat urine has been developed. urine samples, extracted with a protein precipitation procedure were separated on a c18 column using a mixture of water (containing 0.1% formic acid) and acetonitrile (30:70, v/v) as mobile phase. the detection was performed via ms with electrospray ionization interface in positive selected reaction monitoring (srm) mode. one urine sample after administration was selected as ‹standard›. the method validation was carried out according to a conventional method which was calibrated by authentic standards. the fully validated method was applied to the pharmacokinetic study of 2,9-demethyljateorhizine-3-sulfate, 13-methoxyjateorhizine-3-glucoronide and 6-methyljateorhizine-5-glucoronide in rat urine. the results could provide evidence to explain the combination of rhizome coptidis and evodiae fructus in terms of elimination.

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منابع مشابه

Relative Determination Approach to the Metabolites of Protoberberine Alkaloids in Rat Urine by Liquid Chromatography Tandem Mass Spectrometry for the Comparative Studies on Rhizome coptidis and Zuojinwan Preparation

The lack of authentic standards has limited the quantitative analysis of herbal drugs in biological samples. The present work demonstrated a practicable strategy for the assay of herbs and their metabolites independent of authentic standards. A liquid chromatography–electrospray ionization–mass spectrometry (LC–ESI–MS) method for the qualitative and quantitative determination of the metabolites...

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Relative Determination Approach to the Metabolites of Protoberberine Alkaloids in Rat Urine by Liquid Chromatography Tandem Mass Spectrometry for the Comparative Studies on Rhizome coptidis and Zuojinwan Preparation

The lack of authentic standards has limited the quantitative analysis of herbal drugs in biological samples. The present work demonstrated a practicable strategy for the assay of herbs and their metabolites independent of authentic standards. A liquid chromatography–electrospray ionization–mass spectrometry (LC–ESI–MS) method for the qualitative and quantitative determination of the metabolites...

متن کامل

Relative Determination Approach to the Metabolites of Protoberberine Alkaloids in Rat Urine by Liquid Chromatography Tandem Mass Spectrometry for the Comparative Studies on Rhizome coptidis and Zuojinwan Preparation

The lack of authentic standards has limited the quantitative analysis of herbal drugs in biological samples. The present work demonstrated a practicable strategy for the assay of herbs and their metabolites independent of authentic standards. A liquid chromatography- electrospray ionization-mass spectrometry (LC-ESI-MS) method for the qualitative and quantitative determination of the metabolite...

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Comparative Pharmacokinetics of Berberine, Palmatine and Jatrorrhizine in Rat Plasma after Oral Administration of Rhizoma coptidis and Zuojinwan Using Liquid Chromatography-Tandem Mass Spectrometry

A selective and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the determination of berberine, palmatine and jatrorrhizine in rat plasma. Target compounds, together with the internal standard (metronidazole), were extracted from rat plasma samples by protein precipitation with acetonitrile-methanol (1:2, v/v). Chromatography was carried out using a ...

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Comparative Pharmacokinetics of Berberine, Palmatine and Jatrorrhizine in Rat Plasma after Oral Administration of Rhizoma coptidis and Zuojinwan Using Liquid Chromatography-Tandem Mass Spectrometry

A selective and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the determination of berberine, palmatine and jatrorrhizine in rat plasma. Target compounds, together with the internal standard (metronidazole), were extracted from rat plasma samples by protein precipitation with acetonitrile-methanol (1:2, v/v). Chromatography was carried out using a ...

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Characterization and determination of the alkaloid metabolites of Evodiae fructus in rat urine by liquid chromatography-tandem mass spectrometry detection.

The lack of authentic standards limits the quantitative analysis of herbal drugs in biological samples. This present work demonstrated a practicable assay of herbs and their metabolites independent of the availability of authentic standards. A liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) method for the qualitative and quantitative determination of the metabolites ...

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عنوان ژورنال:
the iranian journal of pharmaceutical research

جلد ۱۱، شماره ۴، صفحات ۱۲۶۵-۱۲۷۳

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